When will plasmid construction end?
So I have been studying in the lab for a month and a half, but it seems like I haven’t progressed with my research project. I am still working on expressing the core of Qcr6. And I won’t know if it is the core portion of the protein or not until I finish with constructing the new plasmid. It has taken over a week before new colonies grew and I’m still not sure if the desired plasmid was successfully transformed or not. I guess staying in the lab until late has taken a toll on me, and it’s hard to keep a positive attitude when things aren’t going well. But, I realized that’s what research is, and when good results finally come then it’s all worth it.
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